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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
02/01/2017 |
Actualizado : |
15/10/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
CASTILLO, A.; GAIERO, P.; LÓPEZ CARRO, B.; VILARO, F. |
Afiliación : |
ALICIA MARIA CASTILLO SALLE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; P. GAIERO, Universidad de la República (UdelaR)/ Facultad de Agronomía; B. LÓPEZ CARRO, SECIF-IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); FRANCISCO LUIS VILARO PAREJA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Gametic embryogenic response in wild diploid Solanum species and its implications for genome sequencing projects and breeding. |
Fecha de publicación : |
2016 |
Fuente / Imprenta : |
Plant Tissue Culture and Biotechnology, 2016, v. 26 (2): 159-173. |
DOI : |
10.3329/ptcb.v26i2.30566 |
Idioma : |
Inglés |
Contenido : |
ABSTRACT.
The anther culture response in Solanum commersonii (2n = 2x = 24, 1EBN) and S. chacoense (2n = 2x = 24, 2EBN), two wild potato germplasm resources was studied to obtain haploid plants. Three accessions from each of the two species and 3200 anthers from each genotype were cultured. Authors assessed different culture media; ascorbic acid, L?cysteine and silver nitrate (AgNO3) were included to prevent browning of anther cultures. Addition of AgNO3, was effective to induce embryogenesis. The clones from S. commersonii showed different embryogenic response to androgenesis. However, the three accessions from S. chacoense did not induce any embryo in the same conditions. Ploidy level of the regenerated clones was estimated by flow cytometry and confirmed by chromosome counts. This is the first report of haploid plants obtained from anther culture in S. commersonii, with important implications in sequencing efforts and potato breeding. |
Palabras claves : |
ANTHER CULTURE; DOUBLE HAPLOID; FLOW CYTOMETRY; HAPLOID. |
Thesagro : |
GERMOPLASMA; PAPA; SOLANUM COMMERSONII. |
Asunto categoría : |
-- |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/13501/1/30566-Article-Text-109802-1-10-20161210.pdf
https://www.banglajol.info/index.php/PTCB/article/view/30566
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Marc : |
LEADER 01724naa a2200253 a 4500 001 1056407 005 2019-10-15 008 2016 bl uuuu u00u1 u #d 024 7 $a10.3329/ptcb.v26i2.30566$2DOI 100 1 $aCASTILLO, A. 245 $aGametic embryogenic response in wild diploid Solanum species and its implications for genome sequencing projects and breeding.$h[electronic resource] 260 $c2016 520 $aABSTRACT. The anther culture response in Solanum commersonii (2n = 2x = 24, 1EBN) and S. chacoense (2n = 2x = 24, 2EBN), two wild potato germplasm resources was studied to obtain haploid plants. Three accessions from each of the two species and 3200 anthers from each genotype were cultured. Authors assessed different culture media; ascorbic acid, L?cysteine and silver nitrate (AgNO3) were included to prevent browning of anther cultures. Addition of AgNO3, was effective to induce embryogenesis. The clones from S. commersonii showed different embryogenic response to androgenesis. However, the three accessions from S. chacoense did not induce any embryo in the same conditions. Ploidy level of the regenerated clones was estimated by flow cytometry and confirmed by chromosome counts. This is the first report of haploid plants obtained from anther culture in S. commersonii, with important implications in sequencing efforts and potato breeding. 650 $aGERMOPLASMA 650 $aPAPA 650 $aSOLANUM COMMERSONII 653 $aANTHER CULTURE 653 $aDOUBLE HAPLOID 653 $aFLOW CYTOMETRY 653 $aHAPLOID 700 1 $aGAIERO, P. 700 1 $aLÓPEZ CARRO, B. 700 1 $aVILARO, F. 773 $tPlant Tissue Culture and Biotechnology, 2016$gv. 26 (2): 159-173.
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Registro original : |
INIA Las Brujas (LB) |
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha actual : |
29/10/2014 |
Actualizado : |
04/04/2018 |
Tipo de producción científica : |
Trabajos en Congresos/Conferencias |
Autor : |
CASTRO, M.; BERGER, A.; VIEGA, L.; IBAÑEZ, V.; OTERO, A.; VAZQUEZ, D.; SASTRE, M.; GASO, D. |
Afiliación : |
MARINA CASTRO DERENYI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANDRES GUSTAVO BERGER RICCA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; VILFREDO ARIEL IBAÑEZ ALVEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ALVARO RICARDO OTERO CAMA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DANIEL VAZQUEZ PEYRONEL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MAURICIO SASTRE CHARLO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DEBORAH VIVIANA GASO MELGAR, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Efecto del anegamiento en diferentes estados fenológicos de trigo y cebada. |
Fecha de publicación : |
2011 |
Fuente / Imprenta : |
In: CONGRESSO BRASILEIRO DE FISIOLOGIA VEGETAL 13., REUNIÃO LATINO-AMERICANA DE FISIOLOGIA VEGETAL, 14.,"Mudanças Climáticas Globais: Do gene à planta",Búzios. Río de Janeiro. Livro de resumos. Búzios: Sociedade Brasileira de Fisiologia Vegetal, 2011. |
ISSN : |
1677-0420 |
Idioma : |
Español |
Thesagro : |
FENOLOGÍA. |
Asunto categoría : |
F01 Cultivo |
Marc : |
LEADER 00756nam a2200205 a 4500 001 1051362 005 2018-04-04 008 2011 bl uuuu u0uu1 u #d 022 $a1677-0420 100 1 $aCASTRO, M. 245 $aEfecto del anegamiento en diferentes estados fenológicos de trigo y cebada. 260 $aIn: CONGRESSO BRASILEIRO DE FISIOLOGIA VEGETAL 13., REUNIÃO LATINO-AMERICANA DE FISIOLOGIA VEGETAL, 14.,"Mudanças Climáticas Globais: Do gene à planta",Búzios. Río de Janeiro. Livro de resumos. Búzios: Sociedade Brasileira de Fisiologia Vegetal$c2011 650 $aFENOLOGÍA 700 1 $aBERGER, A. 700 1 $aVIEGA, L. 700 1 $aIBAÑEZ, V. 700 1 $aOTERO, A. 700 1 $aVAZQUEZ, D. 700 1 $aSASTRE, M. 700 1 $aGASO, D.
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